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Do not aliquot the antibody. NOTE : Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Western Blotting Application Solutions Kit.

NOTE : Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.

It should be noted that for the best possible results a fresh blot is always recommended.

Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting Gillinghan.

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This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. View letter.

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